验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84047-1-RR targets APOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | apolipoprotein B (including Ag(x) antigen) |
别名 | Apolipoprotein B, ApoB48, ApoB100, Apo B-100, Apo B |
计算分子量 | 516 kDa |
观测分子量 | 150-250 kDa, 400-520 kDa |
GenBank蛋白编号 | NM_000384 |
基因名称 | APOB |
Gene ID (NCBI) | 338 |
RRID | AB_3671614 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P04114 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).
实验方案
Product Specific Protocols | |
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WB protocol for APOB antibody 84047-1-RR | Download protocol |
IHC protocol for APOB antibody 84047-1-RR | Download protocol |
IF protocol for APOB antibody 84047-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |