CoraLite® Plus 488-conjugated APOB Recombinant antibody
APOB Uni-rAbTM Recombinant Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240884H4
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse liver tissue |
For other applications, we recommend the unconjugated version of this antibody, 84047-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84047 targets APOB in IF-P applications and shows reactivity with human, mouse samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | apolipoprotein B (including Ag(x) antigen) |
| Synonyms | |
| Calculated Molecular Weight | 516 kDa |
| Observed Molecular Weight | 150-250 kDa, 400-520 kDa |
| GenBank Accession Number | NM_000384 |
| Gene Symbol | APOB |
| Gene ID (NCBI) | 338 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04114 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 APOB antibody CL488-84047 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |