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CoraLite® Plus 488-conjugated APOD Monoclonal antibody

APOD Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C6D10

Cat no : CL488-66215

Print datasheet

Synonyms

apolipoprotein D, Apo-D, Apo D, 1C6D10

验证数据展示

  • IF Staining of HeLa using CL488-66215

    IF Staining of HeLa using CL488-66215

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 APOD antibody (CL488-66215, Clone: 1C6D10 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66215

    FC experiment of HepG2 using CL488-66215

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human APOD (CL488-66215, Clone:1C6D10) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66215-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66215 targets APOD in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen APOD fusion protein Ag21422 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name apolipoprotein D
Synonyms apolipoprotein D, Apo-D, Apo D, 1C6D10
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC007402
Gene Symbol APOD
Gene ID (NCBI) 347
RRIDAB_2919301
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05090
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 APOD antibody CL488-66215Download protocol
Standard Protocols
Click here to view our Standard Protocols