• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

APOE Polyclonal antibody

APOE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30535-1-AP

Print datasheet

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

验证数据展示

  • IF Staining of HepG2 using 30535-1-AP

    IF Staining of HepG2 using 30535-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (30535-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human skin cancer using 30535-1-AP

    IHC staining of human skin cancer using 30535-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30535-1-AP (APOE antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 30535-1-AP

    IHC staining of human skin cancer using 30535-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30535-1-AP (APOE antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30535-1-AP

    WB analysis using 30535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30535-1-AP (APOE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 30535-1-AP

    WB analysis of HepG2 using 30535-1-AP

    WB result of APOE antibody (30535-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells.

  • IF Staining of mouse brain using 30535-1-AP

    IF Staining of mouse brain using 30535-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using APOE antibody (30535-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30535-1-AP targets APOE in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Apolipoprotein E
Synonyms ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E
Calculated Molecular Weight 36 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC003557
Gene Symbol APOE
Gene ID (NCBI) 348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02649
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

实验方案

Product Specific Protocols
WB protocol for APOE antibody 30535-1-APDownload protocol
IHC protocol for APOE antibody 30535-1-APDownload protocol
IF protocol for APOE antibody 30535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols