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验证数据展示

  • IF Staining of HepG2 using 30535-1-AP

    IF Staining of HepG2 using 30535-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (30535-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human skin cancer using 30535-1-AP

    IHC staining of human skin cancer using 30535-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30535-1-AP (APOE antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 30535-1-AP

    IHC staining of human skin cancer using 30535-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30535-1-AP (APOE antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30535-1-AP

    WB analysis using 30535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30535-1-AP (APOE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 30535-1-AP

    WB analysis of HepG2 using 30535-1-AP

    WB result of APOE antibody (30535-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells.

  • IF Staining of mouse brain using 30535-1-AP

    IF Staining of mouse brain using 30535-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using APOE antibody (30535-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

APOE Polyclonal antibody

APOE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 30535-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30535-1-AP targets APOE in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Apolipoprotein E
别名 ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E
计算分子量 36 kDa
观测分子量35 kDa
GenBank蛋白编号BC003557
基因名称 APOE
Gene ID (NCBI) 348
RRIDAB_3086354
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02649
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

实验方案

Product Specific Protocols
WB protocol for APOE antibody 30535-1-APDownload protocol
IHC protocol for APOE antibody 30535-1-APDownload protocol
IF protocol for APOE antibody 30535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols