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验证数据展示

  • WB analysis of human plasma using 66830-1-Ig (same clone as 66830-1-PBS)

    WB analysis of human plasma using 66830-1-Ig (same clone as 66830-1-PBS)

    human plasma was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at a range of dilutions from 1:5000 to 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

    WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    IHC staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66830-1-Ig (APOE antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    WB analysis of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    WB result of APOE antibody (66830-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

    WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    IF Staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    IF Staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    IF Staining of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    FC experiment of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug APOE Monoclonal antibody (66830-1-Ig, Clone:1B2C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

APOE Monoclonal antibody, PBS Only

APOE Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 66830-1-PBS

产品说明书

CloneNo. 1B2C9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66830-1-PBS targets APOE in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 APOE fusion protein Ag28186 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Apolipoprotein E
别名 ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E
计算分子量 36 kDa
观测分子量 34-36 kDa
GenBank蛋白编号BC003557
基因名称 APOE
Gene ID (NCBI) 348
RRIDAB_2882173
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02649
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.