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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84775-6-RR (same clone as 84775-6-PBS)

    WB analysis using 84775-6-RR (same clone as 84775-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84775-6-RR (APOE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84775-6-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84775-6-RR (same clone as 84775-6-PBS)

    FC experiment of HepG2 using 84775-6-RR (same clone as 84775-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug APOE Recombinant antibody (84775-6-RR, Clone:242310B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84775-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84775-6-RR

    Affinity and Kinetic Characterization of 84775-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84775-6-RR against Human APOE were performed. The affinity constant is below 1 pM.

APOE Recombinant antibody, PBS Only

APOE Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84775-6-PBS

Print datasheet

CloneNo. 242310B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84775-6-PBS targets APOE in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Apolipoprotein E
Synonyms ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 34-38 kDa
GenBank Accession NumberBC003557
Gene Symbol APOE
Gene ID (NCBI) 348
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02649
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).