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验证数据展示

  • IF Staining of HepG2 using CL488-66124

    IF Staining of HepG2 using CL488-66124

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 APOL1-Specific antibody (CL488-66124, Clone: 1G12D11 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated APOL1-Specific Monoclonal antibody

APOL1-Specific Monoclonal Antibody for IF/ICC
Cat No. CL488-66124

Print datasheet

CloneNo. 1G12D11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOL1 Ready-To-Use IHC Kit. APOL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66124-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66124 targets APOL1-Specific in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen APOL1-Specific fusion protein Ag2016 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name apolipoprotein L, 1
Synonyms APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC017331
Gene Symbol APOL1
Gene ID (NCBI) 8542
RRIDAB_2883265
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14791
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 APOL1-Specific antibody CL488-66124Download protocol
Standard Protocols
Click here to view our Standard Protocols