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验证数据展示

  • WB analysis using 28591-1-AP

    WB analysis using 28591-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28591-1-AP (APOL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

APOL3 Polyclonal antibody

APOL3 Polyclonal Antibody for WB, ELISA
Cat No. 28591-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

ApoL III, APOL3, APOLIII, Apolipoprotein L III, apolipoprotein L, 3, Apolipoprotein L3, CG12 1, CG12_1, TNF inducible protein CG12 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, BxPC-3 cells, HCT 116 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28591-1-AP targets APOL3 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 APOL3 fusion protein Ag29687 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein L, 3
别名 ApoL III, APOL3, APOLIII, Apolipoprotein L III, apolipoprotein L, 3, Apolipoprotein L3, CG12 1, CG12_1, TNF inducible protein CG12 1
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号NM_145640
基因名称 APOL3
Gene ID (NCBI) 80833
RRIDAB_3669663
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95236
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein L3(APOL3) is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. APOL3 is localized in the cytoplasm and plays a central role in cholesterol transport, affecting the movement of lipids, including cholesterol, and allowing the binding of lipids to organelles.

实验方案

Product Specific Protocols
WB protocol for APOL3 antibody 28591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols