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验证数据展示

  • IF Staining of HepG2 using CL488-19885

    IF Staining of HepG2 using CL488-19885

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 APPL1 antibody (CL488-19885) at dilution of 1:200, CL594-phalloidin (red).

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CoraLite® Plus 488-conjugated APPL1 Polyclonal antibody

APPL1 Polyclonal Antibody for IF/ICC
Cat No. CL488-19885

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APPL1 Ready-To-Use IHC Kit. APPL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19885-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19885 targets APPL1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 APPL1 fusion protein Ag13703 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
别名 APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428
计算分子量 709 aa, 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC028599
基因名称 APPL1
Gene ID (NCBI) 26060
RRIDAB_3084028
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKG1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 APPL1 antibody CL488-19885Download protocol
Standard Protocols
Click here to view our Standard Protocols