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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated APPL1 Polyclonal antibody

APPL1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19885

Print datasheet

Synonyms

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428

验证数据展示

  • IF Staining of HepG2 using CL488-19885

    IF Staining of HepG2 using CL488-19885

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 APPL1 antibody (CL488-19885) at dilution of 1:200, CL594-phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APPL1 Ready-To-Use IHC Kit. APPL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19885-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19885 targets APPL1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen APPL1 fusion protein Ag13703 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Synonyms APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428
Calculated Molecular Weight 709 aa, 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC028599
Gene Symbol APPL1
Gene ID (NCBI) 26060
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKG1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 APPL1 antibody CL488-19885Download protocol
Standard Protocols
Click here to view our Standard Protocols