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验证数据展示

  • IF Staining of mouse kidney using CL488-20333

    IF Staining of mouse kidney using CL488-20333

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite® Plus 488 AQP1 antibody (CL488-20333) at dilution of 1:200.

  • IF Staining of human breast cancer using CL488-20333

    IF Staining of human breast cancer using CL488-20333

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 AQP1 antibody (CL488-20333) at dilution of 1:200.

CoraLite® Plus 488-conjugated AQP1 Polyclonal antibody

AQP1 Polyclonal Antibody for IF-P
Cat No. CL488-20333

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse kidney tissue, human breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy AQP1 Ready-To-Use IHC Kit. AQP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20333-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20333 targets AQP1 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 AQP1 fusion protein Ag14093 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aquaporin 1 (Colton blood group)
别名 AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel
计算分子量 269 aa, 29 kDa
观测分子量 25-28 kDa, 40-45 kDa
GenBank蛋白编号BC022486
基因名称 AQP1
Gene ID (NCBI) 358
RRIDAB_2919170
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29972
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AQP1 antibody CL488-20333Download protocol
Standard Protocols
Click here to view our Standard Protocols