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验证数据展示

  • IF Staining of human kidney using CL488-66805

    IF Staining of human kidney using CL488-66805

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 AQP1 antibody (CL488-66805, Clone: 2E5D6 ) at dilution of 1:200.

  • IF Staining of human kidney using CL488-66805

    IF Staining of human kidney using CL488-66805

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 AQP1 antibody (CL488-66805, Clone: 2E5D6 ) at dilution of 1:400, CoraLite®594 Podocalyxin antibody (CL594-18150, red).

CoraLite® Plus 488-conjugated AQP1 Monoclonal antibody

AQP1 Monoclonal Antibody for IF-P
Cat No. CL488-66805

Print datasheet

CloneNo. 2E5D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF-P

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy AQP1 Ready-To-Use IHC Kit. AQP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66805-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66805 targets AQP1 in IF-P applications and shows reactivity with Human, pig samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, pig
Immunogen AQP1 fusion protein Ag14093 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aquaporin 1 (Colton blood group)
Synonyms AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel
Calculated Molecular Weight 269 aa, 29 kDa
Observed Molecular Weight 28 kDa, 38-40 kDa
GenBank Accession NumberBC022486
Gene Symbol AQP1
Gene ID (NCBI) 358
RRIDAB_2919375
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP29972
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AQP1 antibody CL488-66805Download protocol
Standard Protocols
Click here to view our Standard Protocols