验证数据展示
经过测试的应用
| Positive IF-P detected in | human kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy AQP1 Ready-To-Use IHC Kit. AQP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66805-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66805 targets AQP1 in IF-P applications and shows reactivity with Human, pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, pig |
| 免疫原 | AQP1 fusion protein Ag14093 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aquaporin 1 (Colton blood group) |
| 别名 | AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel |
| 计算分子量 | 269 aa, 29 kDa |
| 观测分子量 | 28 kDa, 38-40 kDa |
| GenBank蛋白编号 | BC022486 |
| 基因名称 | AQP1 |
| Gene ID (NCBI) | 358 |
| RRID | AB_2919375 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P29972 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AQP1 antibody CL488-66805 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |