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验证数据展示

  • WB analysis using 68448-1-Ig

    WB analysis using 68448-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat spinal cord using 68448-1-Ig

    WB analysis of rat spinal cord using 68448-1-Ig

    rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68448-1-Ig

    IHC staining of mouse brain using 68448-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68448-1-Ig

    IHC staining of mouse brain using 68448-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Aquaporin 4 Monoclonal antibody

Aquaporin 4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68448-1-Ig

产品说明书

CloneNo. 3G7D3
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, ELISA

别名

AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, rat spinal cord tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68448-1-Ig targets Aquaporin 4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human, rat
免疫原 Aquaporin 4 fusion protein Ag9830 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Aquaporin 4
别名 AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4
计算分子量 323 aa, 35 kDa
观测分子量35-37 kDa
GenBank蛋白编号BC022286
基因名称 Aquaporin 4
Gene ID (NCBI) 361
RRIDAB_3085160
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55087
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for Aquaporin 4 antibody 68448-1-IgDownload protocol
IHC protocol for Aquaporin 4 antibody 68448-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,ratWB

Front Pharmacol

Eleutheroside B alleviates oxidative stress and neuroinflammation by inhibiting the JAK2/STAT3 signaling pathway in a rat high altitude cerebral edema model

Authors - Yacong He
View Article