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Aquaporin 4 Monoclonal antibody

Aquaporin 4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G7D3

Cat no : 68448-1-Ig

Print datasheet

Synonyms

AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4

验证数据展示

  • WB analysis using 68448-1-Ig

    WB analysis using 68448-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat spinal cord using 68448-1-Ig

    WB analysis of rat spinal cord using 68448-1-Ig

    rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68448-1-Ig

    IHC staining of mouse brain using 68448-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68448-1-Ig

    IHC staining of mouse brain using 68448-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inpig brain tissue, rat spinal cord tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68448-1-Ig targets Aquaporin 4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Aquaporin 4 fusion protein Ag9830 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Aquaporin 4
Synonyms AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4
Calculated Molecular Weight 323 aa, 35 kDa
Observed Molecular Weight35-37 kDa
GenBank Accession NumberBC022286
Gene Symbol Aquaporin 4
Gene ID (NCBI) 361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55087
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for Aquaporin 4 antibody 68448-1-IgDownload protocol
IHC protocol for Aquaporin 4 antibody 68448-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols