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验证数据展示

  • WB analysis using 68448-1-Ig (same clone as 68448-1-PBS)

    WB analysis using 68448-1-Ig (same clone as 68448-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

  • WB analysis of rat spinal cord using 68448-1-Ig (same clone as 68448-1-PBS)

    WB analysis of rat spinal cord using 68448-1-Ig (same clone as 68448-1-PBS)

    rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68448-1-Ig (same clone as 68448-1-PBS)

    IHC staining of mouse brain using 68448-1-Ig (same clone as 68448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68448-1-Ig (same clone as 68448-1-PBS)

    IHC staining of mouse brain using 68448-1-Ig (same clone as 68448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

Aquaporin 4 Monoclonal antibody, PBS Only

Aquaporin 4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68448-1-PBS

产品说明书

CloneNo. 3G7D3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, Indirect ELISA

别名

AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68448-1-PBS targets Aquaporin 4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Aquaporin 4 fusion protein Ag9830 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Aquaporin 4
别名 AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4
计算分子量 323 aa, 35 kDa
观测分子量35-37 kDa
GenBank蛋白编号BC022286
基因名称 Aquaporin 4
Gene ID (NCBI) 361
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55087
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.