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AQP5 Polyclonal antibody, PBS Only

AQP5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20334-1-PBS

Print datasheet

Synonyms

aquaporin 5, Aquaporin-5, aquaporin5, AQP-5, AQP 5

验证数据展示

  • WB analysis using 20334-1-AP (same clone as 20334-1-PBS)

    WB analysis using 20334-1-AP (same clone as 20334-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20334-1-AP (AQP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

    IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AQP5 antibody included
  • IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

    IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AQP5 antibody included
  • IF Staining of rat lung using 20334-1-AP (same clone as 20334-1-PBS)

    IF Staining of rat lung using 20334-1-AP (same clone as 20334-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AQP5 antibody (20334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AQP5 Ready-To-Use IHC Kit. AQP5 primary antibody included.

产品信息

20334-1-PBS targets AQP5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AQP5 fusion protein Ag14103 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aquaporin 5
Synonyms aquaporin 5, Aquaporin-5, aquaporin5, AQP-5, AQP 5
Calculated Molecular Weight 265 aa, 28 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC032946
Gene Symbol AQP5
Gene ID (NCBI) 362
RRIDAB_2918066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55064
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AQP5 is a member of aquaporins (AQPs) which are membrane proteins functioning as water channels and involved in the bidirectional transfer of water and small solutes across cell membranes. AQP5 is widely expressed including digestive, renal, respiratory, and reproductive systems. AQP5 overexpression in cancer cells and tumor tissues has been extensively reported. Recently AQP5 has been identified as a marker for adult pyloric stem cells.