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验证数据展示

  • WB analysis using 20334-1-AP (same clone as 20334-1-PBS)

    WB analysis using 20334-1-AP (same clone as 20334-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20334-1-AP (AQP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

    IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AQP5 antibody included
  • IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

    IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AQP5 antibody included
  • IF Staining of rat lung using 20334-1-AP (same clone as 20334-1-PBS)

    IF Staining of rat lung using 20334-1-AP (same clone as 20334-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AQP5 antibody (20334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

AQP5 Polyclonal antibody, PBS Only

AQP5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 20334-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

aquaporin 5, Aquaporin-5, aquaporin5, AQP-5, AQP 5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AQP5 Ready-To-Use IHC Kit. AQP5 primary antibody included.

产品信息

20334-1-PBS targets AQP5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AQP5 fusion protein Ag14103 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aquaporin 5
别名 aquaporin 5, Aquaporin-5, aquaporin5, AQP-5, AQP 5
计算分子量 265 aa, 28 kDa
观测分子量27 kDa
GenBank蛋白编号BC032946
基因名称 AQP5
Gene ID (NCBI) 362
RRIDAB_2918066
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55064
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AQP5 is a member of aquaporins (AQPs) which are membrane proteins functioning as water channels and involved in the bidirectional transfer of water and small solutes across cell membranes. AQP5 is widely expressed including digestive, renal, respiratory, and reproductive systems. AQP5 overexpression in cancer cells and tumor tissues has been extensively reported. Recently AQP5 has been identified as a marker for adult pyloric stem cells.