验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy ARC Ready-To-Use IHC Kit. ARC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66550-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66550 targets ARC in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse, Rat, Pig, rabbit |
| Immunogen | ARC fusion protein Ag9445 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | activity-regulated cytoskeleton-associated protein |
| Synonyms | ARC, ARC/ARG3.1, Arg3.1, KIAA0278 |
| Calculated Molecular Weight | 396 aa, 45 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC012321 |
| Gene Symbol | ARC |
| Gene ID (NCBI) | 23237 |
| RRID | AB_2934733 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q7LC44 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 ARC antibody CL594-66550 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |