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验证数据展示

  • WB analysis using 68204-1-Ig

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68204-1-Ig

    WB analysis of A549 using 68204-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68204-1-Ig

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68204-1-Ig

    WB analysis of HeLa using 68204-1-Ig

    WB result of ARF4 antibody (68204-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells.

  • WB analysis using 68204-1-Ig

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 68204-1-Ig

    IHC staining of human stomach cancer using 68204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARF4 antibody included
  • IHC staining of human stomach cancer using 68204-1-Ig

    IHC staining of human stomach cancer using 68204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARF4 antibody included
  • IHC staining of human liver using 68204-1-Ig

    IHC staining of human liver using 68204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARF4 antibody included
  • IHC staining of human pancreas cancer using 68204-1-Ig

    IHC staining of human pancreas cancer using 68204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARF4 antibody included
  • IF Staining of HepG2 using 68204-1-Ig

    IF Staining of HepG2 using 68204-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARF4 antibody (68204-1-Ig, Clone: 2A4B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of K-562 using 68204-1-Ig

    FC experiment of K-562 using 68204-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (68204-1-Ig, Clone:2A4B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

ARF4 Monoclonal antibody

ARF4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 68204-1-Ig

产品说明书

CloneNo. 2A4B8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

ARF2, ADP-ribosylation factor 4, ADP ribosylation factor 4, 2A4B8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, rat brain tissue, A549 cells, mouse brain tissue, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue, human liver tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ARF4 Ready-To-Use IHC Kit. ARF4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68204-1-Ig targets ARF4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ARF4 fusion protein Ag25522 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor 4
别名 ARF2, ADP-ribosylation factor 4, ADP ribosylation factor 4, 2A4B8
计算分子量 180 aa, 21 kDa
观测分子量18 kDa
GenBank蛋白编号BC003364
基因名称 ARF4
Gene ID (NCBI) 378
RRIDAB_2935292
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP18085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

实验方案

Product Specific Protocols
WB protocol for ARF4 antibody 68204-1-IgDownload protocol
IHC protocol for ARF4 antibody 68204-1-IgDownload protocol
IF protocol for ARF4 antibody 68204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols