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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated ARF4 Polyclonal antibody

ARF4 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11673

Print datasheet

Synonyms

ARF4, ADP ribosylation factor 4, ADP-ribosylation factor 4, ARF2

验证数据展示

  • FC experiment of HepG2 using CL647-11673

    FC experiment of HepG2 using CL647-11673

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ARF4 (CL647-11673) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARF4 Ready-To-Use IHC Kit. ARF4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11673-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11673 targets ARF4 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ARF4 fusion protein Ag2272 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor 4
Synonyms ARF4, ADP ribosylation factor 4, ADP-ribosylation factor 4, ARF2
Calculated Molecular Weight 180 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC003364
Gene Symbol ARF4
Gene ID (NCBI) 378
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18085
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 ARF4 antibody CL647-11673Download protocol
Standard Protocols
Click here to view our Standard Protocols