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验证数据展示

  • WB analysis using 84296-4-RR

    WB analysis using 84296-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84296-4-RR (ARFGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 84296-4-RR

    IHC staining of human stomach cancer using 84296-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 84296-4-RR

    IHC staining of human stomach cancer using 84296-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84296-4-RR

    Affinity and Kinetic Characterization of 84296-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84296-4-RR against Human ARFGEF2 were performed. The affinity constant is 0.788 nM.

ARFGEF2 Recombinant antibody

ARFGEF2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84296-4-RR

产品说明书

CloneNo. 241538H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

BIG2, Brefeldin A inhibited GEP 2, ARFGEP2, 241538H5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84296-4-RR targets ARFGEF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ARFGEF2 fusion protein Ag35396 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
别名 BIG2, Brefeldin A inhibited GEP 2, ARFGEP2, 241538H5
观测分子量202 kDa
GenBank蛋白编号BC050449
基因名称 ARFGEF2
Gene ID (NCBI) 10564
RRIDAB_3671837
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6D5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.

实验方案

Product Specific Protocols
WB protocol for ARFGEF2 antibody 84296-4-RRDownload protocol
IHC protocol for ARFGEF2 antibody 84296-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols