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ARH Monoclonal antibody

ARH Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D1B11

Cat no : 66932-2-Ig

Print datasheet

Synonyms

LDLRAP1, FHCB2, FHCB1, ARH2, ARH1

验证数据展示

  • WB analysis using 66932-2-Ig

    WB analysis using 66932-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66932-2-Ig (ARH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HeLa using 66932-2-Ig

    FC experiment of HeLa using 66932-2-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ARH Monoclonal antibody (66932-2-Ig, Clone:1D1B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, THP-1 cells, HSC-T6 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66932-2-Ig targets ARH in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen ARH fusion protein Ag28608 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name low density lipoprotein receptor adaptor protein 1
Synonyms LDLRAP1, FHCB2, FHCB1, ARH2, ARH1
Calculated Molecular Weight 308 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC029770
Gene Symbol ARH
Gene ID (NCBI) 26119
RRIDAB_2882258
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5SW96
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARH antibody 66932-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Leukocyte immunoglobulin-like receptor B1 (LILRB1) protects human multiple myeloma cells from ferroptosis by maintaining cholesterol homeostasis

Authors - Miao Xian
  • KD Validated
View Article