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验证数据展示

  • WB analysis using 22183-1-AP

    WB analysis using 22183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 22183-1-AP

    IHC staining of human ovary cancer using 22183-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGAP21 antibody included
  • IF Staining of HepG2 using 22183-1-AP

    IF Staining of HepG2 using 22183-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARHGAP21 antibody (22183-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

ARHGAP21 Polyclonal antibody

ARHGAP21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22183-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARHGAP21 Ready-To-Use IHC Kit. ARHGAP21 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22183-1-AP targets ARHGAP21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, rat
文献引用反应性human
免疫原 ARHGAP21 fusion protein Ag16515 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GTPase activating protein 21
别名 Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10
计算分子量 1957 aa, 217 kDa
观测分子量 250 kDa
GenBank蛋白编号BC118915
基因名称 ARHGAP21
Gene ID (NCBI) 57584
RRIDAB_2879018
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T5U3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGAP21 antibody 22183-1-APDownload protocol
IHC protocol for ARHGAP21 antibody 22183-1-APDownload protocol
IF protocol for ARHGAP21 antibody 22183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Phosphorylation-dependent recognition of diverse protein targets by the cryptic GK domain of MAGI MAGUKs

Authors - Meng Zhang
  • KD Validated
View Article
humanWB,IF

Elife

PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.

Authors - Arman Javadi
View Article