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  • Featured Product
  • KD/KO Validated

ARHGAP21 Polyclonal antibody

ARHGAP21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22183-1-AP

Print datasheet

Synonyms

Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10

验证数据展示

  • WB analysis using 22183-1-AP

    WB analysis using 22183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 22183-1-AP

    IF Staining of HepG2 using 22183-1-AP

    Immunofluorescent analysis of HepG2 cells using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 22183-1-AP

    IHC staining of human ovary cancer using 22183-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22183-1-AP targets ARHGAP21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen ARHGAP21 fusion protein Ag16515 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 21
Synonyms Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10
Calculated Molecular Weight 1957 aa, 217 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC118915
Gene Symbol ARHGAP21
Gene ID (NCBI) 57584
RRIDAB_2879018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5U3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGAP21 antibody 22183-1-APDownload protocol
IHC protocol for ARHGAP21 antibody 22183-1-APDownload protocol
IF protocol for ARHGAP21 antibody 22183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Phosphorylation-dependent recognition of diverse protein targets by the cryptic GK domain of MAGI MAGUKs

Authors - Meng Zhang
  • KD Validated
View Article
humanWB,IF

Elife

PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.

Authors - Arman Javadi
View Article