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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ARHGAP21 Polyclonal antibody

ARHGAP21 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22183

Print datasheet

Synonyms

Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10

验证数据展示

  • FC experiment of HepG2 using CL488-22183

    FC experiment of HepG2 using CL488-22183

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Arhgap21 Polyclonal Antibody (CL488-22183)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 22183-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22183 targets ARHGAP21 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen ARHGAP21 fusion protein Ag16515 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 21
Synonyms Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10
Calculated Molecular Weight 1957 aa, 217 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC118915
Gene Symbol ARHGAP21
Gene ID (NCBI) 57584
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5U3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ARHGAP21 antibody CL488-22183Download protocol
Standard Protocols
Click here to view our Standard Protocols