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验证数据展示

  • WB analysis using 66480-1-Ig

    WB analysis using 66480-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66480-1-Ig

    WB analysis of HEK-293 using 66480-1-Ig

    WB result of RhoGDI antibody (66480-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells.

  • Featured Product
  • KD/KO Validated

RhoGDI Monoclonal antibody

RhoGDI Monoclonal Antibody for WB, ELISA
Cat No. 66480-1-Ig

Print datasheet

CloneNo. 1B1G9

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Synonyms

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, C6 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy ARHGDIA Ready-To-Use IHC Kit. ARHGDIA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66480-1-Ig targets RhoGDI in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen RhoGDI fusion protein Ag0791 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Rho GDP dissociation inhibitor (GDI) alpha
Synonyms aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC005851
Gene Symbol RhoGDI
Gene ID (NCBI) 396
RRIDAB_2881846
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52565
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.

实验方案

Product Specific Protocols
WB protocol for RhoGDI antibody 66480-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols