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CoraLite®594-conjugated ARHGDIB Monoclonal antibody

ARHGDIB Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E7B7

Cat no : CL594-66054

Print datasheet

Synonyms

验证数据展示

  • IF Staining of human tonsillitis using CL594-66054

    IF Staining of human tonsillitis using CL594-66054

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite®594 ARHGDIB antibody (CL594-66054, Clone: 3E7B7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66054-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66054 targets ARHGDIB in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen ARHGDIB fusion protein Ag9091 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Rho GDP dissociation inhibitor (GDI) beta
Synonyms
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC009200
Gene Symbol ARHGDIB
Gene ID (NCBI) 397
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52566
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

实验方案

Product Specific Protocols
IF protocol for CL594 ARHGDIB antibody CL594-66054Download protocol
Standard Protocols
Click here to view our Standard Protocols