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验证数据展示

  • WB analysis using 68428-1-Ig

    WB analysis using 68428-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 68428-1-Ig

    IF Staining of K-562 using 68428-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ARID3A antibody (68428-1-Ig, Clone: 2B10C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

ARID3A Monoclonal antibody

ARID3A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68428-1-Ig

产品说明书

CloneNo. 2B10C10

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HuH-7 cells, Jurkat cells, K-562 cells, TF-1 cells, HepG2 cells
Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ARID3A Ready-To-Use IHC Kit. ARID3A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68428-1-Ig targets ARID3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ARID3A fusion protein Ag7655 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 AT rich interactive domain 3A (BRIGHT-like)
别名 Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A
计算分子量 63 kDa
观测分子量 75 kDa
GenBank蛋白编号BC060828
基因名称 ARID3A
Gene ID (NCBI) 1820
RRIDAB_3085142
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99856
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).

实验方案

Product Specific Protocols
WB protocol for ARID3A antibody 68428-1-IgDownload protocol
IF protocol for ARID3A antibody 68428-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols