• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ARID3A Monoclonal antibody, PBS Only

ARID3A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B10C10

Cat no : 68428-1-PBS

Print datasheet

Synonyms

Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A

验证数据展示

  • WB analysis using 68428-1-Ig (same clone as 68428-1-PBS)

    WB analysis using 68428-1-Ig (same clone as 68428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 68428-1-Ig (same clone as 68428-1-PBS)

    IF Staining of K-562 using 68428-1-Ig (same clone as 68428-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ARID3A antibody (68428-1-Ig, Clone: 2B10C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ARID3A Ready-To-Use IHC Kit. ARID3A primary antibody included.

产品信息

68428-1-PBS targets ARID3A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ARID3A fusion protein Ag7655 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name AT rich interactive domain 3A (BRIGHT-like)
Synonyms Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC060828
Gene Symbol ARID3A
Gene ID (NCBI) 1820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99856
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).