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ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Guinea Pig / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30332-1-AP

Print datasheet

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

验证数据展示

  • WB analysis using 30332-1-AP

    WB analysis using 30332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30332-1-AP (ARL13B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The second antibody is used against guinea pigs.

  • IP experiment of L02 using 30332-1-AP

    IP experiment of L02 using 30332-1-AP

    IP result of anti-ARL13B (IP:30332-1-AP, 4ug; Detection:30332-1-AP 1:4000) with L02 cells lysate 3080 ug.

  • IF Staining of MDCK using 30332-1-AP

    IF Staining of MDCK using 30332-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ARL13B antibody (30332-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, L02 cells
Positive IP detected inL02 cells
Positive IF/ICC detected inMDCK cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30332-1-AP targets ARL13B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen ARL13B fusion protein Ag12015 种属同源性预测
Host / Isotype Guinea Pig / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor-like 13B
Synonyms ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SXY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.

实验方案

Product Specific Protocols
WB protocol for ARL13B antibody 30332-1-APDownload protocol
IF protocol for ARL13B antibody 30332-1-APDownload protocol
IP protocol for ARL13B antibody 30332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols