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验证数据展示

  • WB analysis using 30332-1-AP

    WB analysis using 30332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30332-1-AP (ARL13B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The second antibody is used against guinea pigs.

  • IP experiment of L02 using 30332-1-AP

    IP experiment of L02 using 30332-1-AP

    IP result of anti-ARL13B (IP:30332-1-AP, 4ug; Detection:30332-1-AP 1:4000) with L02 cells lysate 3080 ug.

  • IF Staining of MDCK using 30332-1-AP

    IF Staining of MDCK using 30332-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ARL13B antibody (30332-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 30332-1-AP

产品说明书

宿主/亚型

Guinea Pig / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, L02 cells
Positive IP detected inL02 cells
Positive IF/ICC detected inMDCK cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30332-1-AP targets ARL13B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 ARL13B fusion protein Ag12015 种属同源性预测
宿主/亚型 Guinea Pig / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 13B
别名 ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B
计算分子量 48 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC094725
基因名称 ARL13B
Gene ID (NCBI) 200894
RRIDAB_3086296
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3SXY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.

实验方案

Product Specific Protocols
WB protocol for ARL13B antibody 30332-1-APDownload protocol
IF protocol for ARL13B antibody 30332-1-APDownload protocol
IP protocol for ARL13B antibody 30332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols