ARL13B Polyclonal antibody
ARL13B Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Guinea Pig / IgG
Reactivity
Human
Applications
WB, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, L02 cells |
Positive IP detected in | L02 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30332-1-AP targets ARL13B in WB, IP, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IP, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | ARL13B fusion protein Ag12015 种属同源性预测 |
Host / Isotype | Guinea Pig / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ADP-ribosylation factor-like 13B |
Synonyms | ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 48-55 kDa |
GenBank Accession Number | BC094725 |
Gene Symbol | ARL13B |
Gene ID (NCBI) | 200894 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q3SXY8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.
实验方案
Product Specific Protocols | |
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WB protocol for ARL13B antibody 30332-1-AP | Download protocol |
IP protocol for ARL13B antibody 30332-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |