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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-17711

Print datasheet

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

验证数据展示

  • IF Staining of MDCK using CL594-17711

    IF Staining of MDCK using CL594-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 ARL13B antibody (CL594-17711) at dilution of 1:200.

  • IF Staining of MDCK using CL594-17711

    IF Staining of MDCK using CL594-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 ARL13B antibody (CL594-17711) at dilution of 1:200.

  • IF Staining of MDCK using CL594-17711

    IF Staining of MDCK using CL594-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-17711 (ARL13B antibody) at dilution of 1:50. Cells were co-stained with CL488-66200 (acetylated Tubulin(Lys40) antibody).

  • IF Staining of hTERT-RPE1 using CL594-17711

    IF Staining of hTERT-RPE1 using CL594-17711

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®594 ARL13B antibody (CL594-17711) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

经过测试的应用

Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 17711-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17711 targets ARL13B in IF/ICC applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, canine
Immunogen ARL13B fusion protein Ag12015 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor-like 13B
Synonyms ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
RRIDAB_2883407
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SXY8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite594-Conjugated ARL13B antibody can be used to mark the cilia(red). Ex: 593nm, Em: 614nm

实验方案

Product Specific Protocols
IF protocol for CL594 ARL13B antibody CL594-17711Download protocol
Standard Protocols
Click here to view our Standard Protocols