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验证数据展示

  • WB analysis using 17711-1-AP

    WB analysis using 17711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 17711-1-AP

    WB analysis of mouse liver using 17711-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH3T3 cells using 17711-1-AP

    IF Staining of NIH3T3 cells using 17711-1-AP

    IF result of anti-ARL13B (17711-1-AP) in NIH3T3 cell by Dr. Sudipto.

  • IF Staining of hTERT-RPE1 using 17711-1-AP

    IF Staining of hTERT-RPE1 using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of MDCK using 17711-1-AP

    IF Staining of MDCK using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • IF Staining of hTERT-RPE1 using 17711-1-AP

    IF Staining of hTERT-RPE1 using 17711-1-AP

    IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.

  • IF Staining of hTERT-RPE1 using 17711-1-AP

    IF Staining of hTERT-RPE1 using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • IF Staining of mouse embryonic fibroblasts using 17711-1-AP

    IF Staining of mouse embryonic fibroblasts using 17711-1-AP

    IF result from Dr. Corbit, Kevin. anti-ARL13B (17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

  • IP experiment of L02 using 17711-1-AP

    IP experiment of L02 using 17711-1-AP

    IP result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.

  • IHC staining of mouse brain using 17711-1-AP

    IHC staining of mouse brain using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17711-1-AP

    IHC staining of mouse brain using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 17711-1-AP

    IF Staining of mouse pancreas using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CoraLite® Plus 488 E-cadherin antibody (CL488-20874, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ARL13BA Polyclonal antibody

ARL13BA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17711-1-AP

产品说明书

引用文献(869)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine and More (6)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

ARL13B, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue, rat kidney tissue
Positive IP detected inL02 cells
Positive IHC detected inmouse brain tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, NIH3T3 cells, MDCK cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17711-1-AP targets ARL13BA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus
免疫原

CatNo: Ag12015

Product name: Recombinant human ARL13B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-321 aa of BC094725

Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 13B
别名 ARL13B, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1
计算分子量 48 kDa
观测分子量 40-48 kDa, 60 kDa
GenBank蛋白编号BC094725
基因名称 ARL13B
Gene ID (NCBI) 200894
RRIDAB_2060867
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3SXY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named ARL2L1, is the Ras superfamily's small ciliary G protein. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B cause Joubert syndrome (JS), an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band will likely represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

实验方案

Product Specific Protocols
IF protocol for ARL13BA antibody 17711-1-APDownload protocol
IHC protocol for ARL13BA antibody 17711-1-APDownload protocol
IP protocol for ARL13BA antibody 17711-1-APDownload protocol
WB protocol for ARL13BA antibody 17711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

The developmental basis of fingerprint pattern formation and variation

Authors - James D Glover
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humanIF

Nat Biotechnol

Guided self-organization and cortical plate formation in human brain organoids.

Authors - Madeline A Lancaster
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mouseIF

Nature

Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.

Authors - David Willnow
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mouseIF

Science

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.

Authors - Avishag Mytlis
View Article
humanIF

Nature

A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.

Authors - Adriana Sánchez-Danés
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mouseIF

Nature

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.

Authors - Matthew B Johnson
View Article
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