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  • Featured Product
  • KD/KO Validated

ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17711-1-AP

Print datasheet

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

验证数据展示

  • WB analysis using 17711-1-AP

    WB analysis using 17711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 17711-1-AP

    WB analysis of mouse liver using 17711-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH3T3 cells using 17711-1-AP

    IF Staining of NIH3T3 cells using 17711-1-AP

    IF result of anti-ARL13B (17711-1-AP) in NIH3T3 cell by Dr. Sudipto.

  • IF Staining of MDCK using 17711-1-AP

    IF Staining of MDCK using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ARL13B antibody (17711-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of hTERT-RPE1 using 17711-1-AP

    IF Staining of hTERT-RPE1 using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of hTERT-RPE1 using 17711-1-AP

    IF Staining of hTERT-RPE1 using 17711-1-AP

    IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.

  • IF Staining of hTERT-RPE1 using 17711-1-AP

    IF Staining of hTERT-RPE1 using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • IF Staining of mouse embryonic fibroblasts using 17711-1-AP

    IF Staining of mouse embryonic fibroblasts using 17711-1-AP

    IF result from Dr. Corbit, Kevin. anti-ARL13B (17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

  • IP experiment of L02 using 17711-1-AP

    IP experiment of L02 using 17711-1-AP

    IP result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.

  • IHC staining of mouse brain using 17711-1-AP

    IHC staining of mouse brain using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17711-1-AP

    IHC staining of mouse brain using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inL02 cells, NIH/3T3 cells, mouse liver tissue, mouse kidney tissue, mouse brain tissue, rat liver tissue, rat kidney tissue
Positive IP detected inL02 cells
Positive IHC detected inmouse brain tissue, human kidney tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, NIH3T3 cells, MDCK cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17711-1-AP targets ARL13B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus
Immunogen ARL13B fusion protein Ag12015 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor-like 13B
Synonyms ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 40-48 kDa, 60 kDa
GenBank Accession NumberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
RRIDAB_2060867
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SXY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

实验方案

Product Specific Protocols
WB protocol for ARL13B antibody 17711-1-APDownload protocol
IHC protocol for ARL13B antibody 17711-1-APDownload protocol
IF protocol for ARL13B antibody 17711-1-APDownload protocol
IP protocol for ARL13B antibody 17711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

The developmental basis of fingerprint pattern formation and variation

Authors - James D Glover
View Article
humanIF

Nat Biotechnol

Guided self-organization and cortical plate formation in human brain organoids.

Authors - Madeline A Lancaster
View Article
mouseIF

Nature

Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.

Authors - David Willnow
View Article
mouseIF

Science

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.

Authors - Avishag Mytlis
View Article
humanIF

Nature

A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.

Authors - Adriana Sánchez-Danés
View Article
mouseIF

Nature

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.

Authors - Matthew B Johnson
View Article