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ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (7)

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17711-1-AP

Print datasheet

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, JBTS8



经过测试的应用

Positive WB detected inmouse liver tissue, NIH/3T3 cells, L02 cells, mouse kidney tissue, rat liver tissue, mouse brain tissue
Positive IP detected inL02 cells
Positive IHC detected inmouse brain tissue, human testis tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, hTERT-RPE1 cells, hTERT-RPE1 cells, NIH3T3 cells, mouse embryonic fibroblasts
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

17711-1-AP targets ARL13B in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications FC, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, canine, chicken, monkey, pig, sheep, Xenopus, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12015
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
Observed molecular weight 40-48 kDa, 66 kDa
GenBank accession numberBC094725
Gene symbol ARL13B
UniprotQ3SXY8
Gene ID (NCBI) 200894
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

实验方案

Product Specific Protocols
WB protocol for ARL13B antibody 17711-1-APDownload protocol
IHC protocol for ARL13B antibody 17711-1-APDownload protocol
IF protocol for ARL13B antibody 17711-1-APDownload protocol
IP protocol for ARL13B antibody 17711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Development

Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines.

Authors - Elizabeth E Capowski
IF

FASEB J

Tubular flow activates magnesium transport in the distal convoluted tubule.

Authors - Eric H J Verschuren
mouseIF

Mol Cells

Loss of Primary Cilia Results in the Development of Cancer in the Murine Thyroid Gland.

Authors - Junguee Lee
ratIF

Sci Rep

Hydrocephalus in a rat model of Meckel Gruber syndrome with a TMEM67 mutation.

Authors - Joon W Shim
ZebrafishIF

Commun Biol

Pharmacological cholesterol depletion disturbs ciliogenesis and ciliary function in developing zebrafish.

Authors - Lars D Maerz
mouseIF

Neuron

HDAC1 and HDAC2 Regulate Intermediate Progenitor Positioning to Safeguard Neocortical Development.

Authors - Tianxiang Tang