验证数据展示
经过测试的应用
Positive WB detected in | L02 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue, rat kidney tissue |
Positive IP detected in | L02 cells |
Positive IHC detected in | mouse brain tissue, human kidney tissue, human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | hTERT-RPE1 cells, NIH3T3 cells, MDCK cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17711-1-AP targets ARL13B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse, rat, canine |
文献引用反应性 | human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus |
免疫原 | ARL13B fusion protein Ag12015 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ADP-ribosylation factor-like 13B |
别名 | ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1, ARL2-like protein 1 |
计算分子量 | 48 kDa |
观测分子量 | 40-48 kDa, 60 kDa |
GenBank蛋白编号 | BC094725 |
基因名称 | ARL13B |
Gene ID (NCBI) | 200894 |
RRID | AB_2060867 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q3SXY8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL13B, also named ARL2L1, is the Ras superfamily's small ciliary G protein. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B cause Joubert syndrome (JS), an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band will likely represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
实验方案
Product Specific Protocols | |
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WB protocol for ARL13B antibody 17711-1-AP | Download protocol |
IHC protocol for ARL13B antibody 17711-1-AP | Download protocol |
IF protocol for ARL13B antibody 17711-1-AP | Download protocol |
IP protocol for ARL13B antibody 17711-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Biotechnol Guided self-organization and cortical plate formation in human brain organoids. | ||
Nature Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche. | ||
Science Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium. | ||
Nature A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy. | ||
Nature Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. |