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ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17711-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine and More (6)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1, ARL2-like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive WB detected inL02 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue, rat kidney tissue
Positive IP detected inL02 cells
Positive IHC detected inmouse brain tissue, human kidney tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, NIH3T3 cells, MDCK cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17711-1-AP targets ARL13B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus
免疫原 ARL13B fusion protein Ag12015 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 13B
别名 ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1, ARL2-like protein 1
计算分子量 48 kDa
观测分子量 40-48 kDa, 60 kDa
GenBank蛋白编号BC094725
基因名称 ARL13B
Gene ID (NCBI) 200894
RRIDAB_2060867
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3SXY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named ARL2L1, is the Ras superfamily's small ciliary G protein. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B cause Joubert syndrome (JS), an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band will likely represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

实验方案

Product Specific Protocols
WB protocol for ARL13B antibody 17711-1-APDownload protocol
IHC protocol for ARL13B antibody 17711-1-APDownload protocol
IF protocol for ARL13B antibody 17711-1-APDownload protocol
IP protocol for ARL13B antibody 17711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

The developmental basis of fingerprint pattern formation and variation

Authors - James D Glover
humanIF

Nat Biotechnol

Guided self-organization and cortical plate formation in human brain organoids.

Authors - Madeline A Lancaster
mouseIF

Nature

Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.

Authors - David Willnow
mouseIF

Science

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.

Authors - Avishag Mytlis
humanIF

Nature

A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.

Authors - Adriana Sánchez-Danés
mouseIF

Nature

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.

Authors - Matthew B Johnson
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