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验证数据展示

  • IF Staining of hTERT-RPE1 using CL555-17711

    IF Staining of hTERT-RPE1 using CL555-17711

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 555 ARL13B antibody (CL555-17711) at dilution of 1:200, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • IF Staining of MDCK using CL555-17711

    IF Staining of MDCK using CL555-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 555 ARL13B antibody (CL555-17711) at dilution of 1:200, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 555-conjugated ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF/ICC
Cat No. CL555-17711

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

IF/ICC

别名

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells
For other applications, we recommend the unconjugated version of this antibody, 17711-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-17711 targets ARL13B in IF/ICC applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, canine
免疫原 ARL13B fusion protein Ag12015 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 13B
别名 ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B
计算分子量 48 kDa
GenBank蛋白编号BC094725
基因名称 ARL13B
Gene ID (NCBI) 200894
RRIDAB_3673405
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ3SXY8
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

实验方案

Product Specific Protocols
IF protocol for CL Plus 555 ARL13B antibody CL555-17711Download protocol
Standard Protocols
Click here to view our Standard Protocols