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验证数据展示

  • IF Staining of MDCK using CL594-17711

    IF Staining of MDCK using CL594-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 ARL13B antibody (CL594-17711) at dilution of 1:200.

  • IF Staining of MDCK using CL594-17711

    IF Staining of MDCK using CL594-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 ARL13B antibody (CL594-17711) at dilution of 1:200.

  • IF Staining of MDCK using CL594-17711

    IF Staining of MDCK using CL594-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-17711 (ARL13B antibody) at dilution of 1:50. Cells were co-stained with CL488-66200 (acetylated Tubulin(Lys40) antibody).

  • IF Staining of hTERT-RPE1 using CL594-17711

    IF Staining of hTERT-RPE1 using CL594-17711

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®594 ARL13B antibody (CL594-17711) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF/ICC
Cat No. CL594-17711

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

IF/ICC

别名

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 17711-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17711 targets ARL13B in IF/ICC applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, canine
免疫原 ARL13B fusion protein Ag12015 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 13B
别名 ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B
计算分子量 48 kDa
GenBank蛋白编号BC094725
基因名称 ARL13B
Gene ID (NCBI) 200894
RRIDAB_2883407
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3SXY8
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite594-Conjugated ARL13B antibody can be used to mark the cilia(red). Ex: 593nm, Em: 614nm

实验方案

Product Specific Protocols
IF protocol for CL594 ARL13B antibody CL594-17711Download protocol
Standard Protocols
Click here to view our Standard Protocols