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CoraLite®594-conjugated ARL2BP Monoclonal antibody

ARL2BP Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G5C2

Cat no : CL594-67316

Print datasheet

Synonyms

RP66, BART1, BART, ARF-like 2-binding protein, ADP-ribosylation factor-like protein 2-binding protein

验证数据展示

  • IF Staining of U2OS using CL594-67316

    IF Staining of U2OS using CL594-67316

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®594 ARL2BP antibody (CL594-67316, Clone: 1G5C2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67316

    FC experiment of HeLa using CL594-67316

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ARL2BP (CL594-67316, Clone:1G5C2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67316-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67316 targets ARL2BP in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen ARL2BP fusion protein Ag29261 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor-like 2 binding protein
Synonyms RP66, BART1, BART, ARF-like 2-binding protein, ADP-ribosylation factor-like protein 2-binding protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC003087
Gene Symbol ARL2BP
Gene ID (NCBI) 23568
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y2Y0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 ARL2BP antibody CL594-67316Download protocol
FC protocol for CL594 ARL2BP antibody CL594-67316Download protocol
Standard Protocols
Click here to view our Standard Protocols