• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • IF Staining of C6 using 68186-1-Ig (same clone as 68186-1-PBS)

    IF Staining of C6 using 68186-1-Ig (same clone as 68186-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 68186-1-Ig (same clone as 68186-1-PBS)

    IF Staining of Neuro-2a using 68186-1-Ig (same clone as 68186-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 68186-1-Ig (same clone as 68186-1-PBS)

    IF Staining of A549 using 68186-1-Ig (same clone as 68186-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68186-1-Ig (same clone as 68186-1-PBS)

    IF Staining of HEK-293 using 68186-1-Ig (same clone as 68186-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

ARL8A/ARL8B Monoclonal antibody, PBS Only

ARL8A/ARL8B Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68186-1-PBS

产品说明书

CloneNo. 2F4C3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

68186-1-PBS targets ARL8A/ARL8B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 ARL8A/ARL8B fusion protein Ag10758 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 8A
别名 ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B
计算分子量 186 aa, 21 kDa
观测分子量18 kDa, 21 kDa
GenBank蛋白编号BC015408
基因名称 ARL8A
Gene ID (NCBI) 127829
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96BM9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A/ARL8B can be recognized by this antibody.