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ARL8B Monoclonal antibody, PBS Only

ARL8B Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G10E2

Cat No : 67891-1-PBS

Print datasheet

Synonyms

ARL10C, ARL8B, FLJ10702, Gie1

验证数据展示

  • WB analysis using 67891-1-Ig (same clone as 67891-1-PBS)

    WB analysis using 67891-1-Ig (same clone as 67891-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67891-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 67891-1-Ig (same clone as 67891-1-PBS)

    WB analysis of LNCaP using 67891-1-Ig (same clone as 67891-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67891-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 67891-1-Ig (same clone as 67891-1-PBS)

    IF Staining of A549 using 67891-1-Ig (same clone as 67891-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8B antibody (67891-1-Ig, Clone: 2G10E2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67891-1-PBS in a different storage buffer formulation.

产品信息

67891-1-PBS targets ARL8B in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen ARL8B fusion protein Ag3711 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor-like 8B
Synonyms ARL10C, ARL8B, FLJ10702, Gie1
Calculated Molecular Weight 186 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC013131
Gene Symbol ARL8B
Gene ID (NCBI) 55207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NVJ2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.