• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ARL8B Monoclonal antibody

ARL8B Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G10E2

Cat no : 67891-1-Ig

Print datasheet

Synonyms

ARL10C, ARL8B, FLJ10702, Gie1

验证数据展示

  • WB analysis using 67891-1-Ig

    WB analysis using 67891-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of LNCaP using 67891-1-Ig

    WB analysis of LNCaP using 67891-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67891-1-Ig

    IF Staining of A549 using 67891-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8B antibody (67891-1-Ig, Clone: 2G10E2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells, pig brain tissue, rat brain tissue
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67891-1-Ig targets ARL8B in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen ARL8B fusion protein Ag3711 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor-like 8B
Synonyms ARL10C, ARL8B, FLJ10702, Gie1
Calculated Molecular Weight 186 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC013131
Gene Symbol ARL8B
Gene ID (NCBI) 55207
RRIDAB_2918647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NVJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

实验方案

Product Specific Protocols
WB protocol for ARL8B antibody 67891-1-IgDownload protocol
IF protocol for ARL8B antibody 67891-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols