• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67891-1-Ig

    WB analysis using 67891-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of LNCaP using 67891-1-Ig

    WB analysis of LNCaP using 67891-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67891-1-Ig

    IF Staining of A549 using 67891-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8B antibody (67891-1-Ig, Clone: 2G10E2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

ARL8B Monoclonal antibody

ARL8B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67891-1-Ig

产品说明书

CloneNo. 2G10E2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

ARL10C, ARL8B, FLJ10702, Gie1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells, pig brain tissue, rat brain tissue
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67891-1-Ig targets ARL8B in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 ARL8B fusion protein Ag3711 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 8B
别名 ARL10C, ARL8B, FLJ10702, Gie1
计算分子量 186 aa, 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC013131
基因名称 ARL8B
Gene ID (NCBI) 55207
RRIDAB_2918647
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NVJ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

实验方案

Product Specific Protocols
WB protocol for ARL8B antibody 67891-1-IgDownload protocol
IF protocol for ARL8B antibody 67891-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols