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验证数据展示

  • WB analysis using CL488-67891

    WB analysis using CL488-67891

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67891 (ARL8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-67891

    FC experiment of HeLa using CL488-67891

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ARL8B (CL488-67891, Clone:2G10E2) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated ARL8B Monoclonal antibody

ARL8B Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-67891
CloneNo. 2G10E2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra)

Synonyms

GIE1, EC:3.6.5.2, ARL10C, ADP-ribosylation factor-like protein 8B, ADP-ribosylation factor-like protein 10C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67891-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67891 targets ARL8B in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ARL8B fusion protein Ag3711 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor-like 8B
Synonyms GIE1, EC:3.6.5.2, ARL10C, ADP-ribosylation factor-like protein 8B, ADP-ribosylation factor-like protein 10C
Calculated Molecular Weight 186 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC013131
Gene Symbol ARL8B
Gene ID (NCBI) 55207
RRIDAB_2923818
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NVJ2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 ARL8B antibody CL488-67891Download protocol
FC protocol for CL Plus 488 ARL8B antibody CL488-67891Download protocol
Standard Protocols
Click here to view our Standard Protocols