验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67891 targets ARL8B in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat, pig |
Immunogen | ARL8B fusion protein Ag3711 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | ADP-ribosylation factor-like 8B |
Synonyms | GIE1, EC:3.6.5.2, ARL10C, ADP-ribosylation factor-like protein 8B, ADP-ribosylation factor-like protein 10C |
Calculated Molecular Weight | 186 aa, 22 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC013131 |
Gene Symbol | ARL8B |
Gene ID (NCBI) | 55207 |
RRID | AB_2923818 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9NVJ2 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 488 ARL8B antibody CL488-67891 | Download protocol |
FC protocol for CL Plus 488 ARL8B antibody CL488-67891 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |