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ARNT,HIF1B Monoclonal antibody, PBS Only

ARNT,HIF1B Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat No : 66732-1-PBS

Print datasheet

Synonyms

ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO

验证数据展示

  • WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

    WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66732-1-Ig (same clone as 66732-1-PBS)

    IF Staining of HepG2 using 66732-1-Ig (same clone as 66732-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.

产品信息

66732-1-PBS targets ARNT,HIF1B in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen ARNT,HIF1B fusion protein Ag5507 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aryl hydrocarbon receptor nuclear translocator
Synonyms ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO
Calculated Molecular Weight 416 aa, 46 kDa, 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC060838
Gene Symbol ARNT
Gene ID (NCBI) 405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27540
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia