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ARNT/HIF-1 beta Monoclonal antibody

ARNT/HIF-1 beta Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66732-1-Ig

Print datasheet

Synonyms

ARNT, HIF-1 beta, HIF1B, 3B12C5, HIF1 beta

验证数据展示

  • WB analysis using 66732-1-Ig

    WB analysis using 66732-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66732-1-Ig

    IF Staining of HepG2 using 66732-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66732-1-Ig

    FC experiment of HeLa using 66732-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT/HIF-1 beta (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, HepG2 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66732-1-Ig targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ARNT/HIF-1 beta fusion protein Ag5507 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aryl hydrocarbon receptor nuclear translocator
Synonyms ARNT, HIF-1 beta, HIF1B, 3B12C5, HIF1 beta
Calculated Molecular Weight 416 aa, 46 kDa, 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC060838
Gene Symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
RRIDAB_2882082
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27540
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

实验方案

Product Specific Protocols
WB protocol for ARNT/HIF-1 beta antibody 66732-1-IgDownload protocol
IF protocol for ARNT/HIF-1 beta antibody 66732-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols