CoraLite®594-conjugated ARNT,HIF1B Monoclonal antibody
ARNT,HIF1B Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3B12C5
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66732-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66732 targets ARNT,HIF1B in IF applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | ARNT,HIF1B fusion protein Ag5507 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | aryl hydrocarbon receptor nuclear translocator |
Synonyms | ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO |
Calculated Molecular Weight | 416 aa, 46 kDa, 87 kDa |
Observed Molecular Weight | 87 kDa |
GenBank Accession Number | BC060838 |
Gene Symbol | ARNT |
Gene ID (NCBI) | 405 |
RRID | AB_2883619 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P27540 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia
实验方案
Product Specific Protocols | |
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IF protocol for CL594 ARNT,HIF1B antibody CL594-66732 | Download protocol |
FC protocol for CL594 ARNT,HIF1B antibody CL594-66732 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |