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验证数据展示

  • WB analysis using 67320-1-Ig

    WB analysis using 67320-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67320-1-Ig (ARPC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 67320-1-Ig

    IHC staining of human appendicitis using 67320-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC1B antibody included
  • IHC staining of mouse spleen using 67320-1-Ig

    IHC staining of mouse spleen using 67320-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC1B antibody included

ARPC1B Monoclonal antibody

ARPC1B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67320-1-Ig

产品说明书

CloneNo. 2C8A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse

应用

WB, IHC, ELISA

别名

ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, THP-1 cells, rat spleen tissues
Positive IHC detected inhuman appendicitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARPC1B Ready-To-Use IHC Kit. ARPC1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67320-1-Ig targets ARPC1B in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 ARPC1B fusion protein Ag29095 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 actin related protein 2/3 complex, subunit 1B, 41kDa
别名 ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC
计算分子量 41 kDa
观测分子量 38 kDa
GenBank蛋白编号BC007555
基因名称 ARPC1B
Gene ID (NCBI) 10095
RRIDAB_2882580
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15143
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARPC1B also known as ARC41, p41-ARC, or p40-ARC, is one of seven subunits of the human Arp2/3 protein complex, that belongs to the SOP2 family. ARPC1B localizes on centrosomes and has a distinct role in centrosomal homeostasis. ARPC1B is both a physiological activator and substrate of Aurora A kinase, and these interactions help to maintain mitotic integrity in mammalian cells. In recent resaeach, ARPC1B is selected as a candidate prediction marker for sensitivity of Choroidal malignant melanomas to radiotherapy.

实验方案

Product Specific Protocols
WB protocol for ARPC1B antibody 67320-1-IgDownload protocol
IHC protocol for ARPC1B antibody 67320-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols