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  • Featured Product
  • KD/KO Validated

ARSB Polyclonal antibody

ARSB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13227-1-AP

Print datasheet

Synonyms

ARSB, arylsulfatase B, ASB, G4S, MPS6

验证数据展示

  • WB analysis of HepG2 using 13227-1-AP

    WB analysis of HepG2 using 13227-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13227-1-AP (ARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13227-1-AP

    IHC staining of human placenta using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 13227-1-AP

    IHC staining of human placenta using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman placenta tissue, human breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13227-1-AP targets ARSB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ARSB fusion protein Ag4083 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name arylsulfatase B
Synonyms ARSB, arylsulfatase B, ASB, G4S, MPS6
Calculated Molecular Weight 413 aa, 46 kDa
Observed Molecular Weight60 kDa, 44 kDa
GenBank Accession NumberBC029051
Gene Symbol ARSB
Gene ID (NCBI) 411
RRIDAB_2227579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15848
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARSB antibody 13227-1-APDownload protocol
IHC protocol for ARSB antibody 13227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Oxid Med Cell Longev

miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling.

Authors - Que Wang
  • KD Validated
View Article
mouseWB

Elife

Chondroitin sulfate proteoglycan 4,6 sulfation regulates sympathetic nerve regeneration after myocardial infarction.

Authors - Matthew R Blake
View Article
mouseWB,IF

Biochem Biophys Rep

Generation of a novel disease model mouse for mucopolysaccharidosis type VI via c. 252T>C human ARSB mutation knock-in

Authors - Kosuke Hosoba
View Article