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ASAH1 Monoclonal antibody

ASAH1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B3C7

Cat no : 67092-2-Ig

Print datasheet

Synonyms

ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC

验证数据展示

  • WB analysis using 67092-2-Ig

    WB analysis using 67092-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 67092-2-Ig

    IF Staining of MCF-7 using 67092-2-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ASAH1 antibody (67092-2-Ig, Clone: 2B3C7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

经过测试的应用

Positive WB detected inpig liver tissue, pig brain tissue, rabbit brain tissue, rat brain tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ASAH1 Ready-To-Use IHC Kit. ASAH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67092-2-Ig targets ASAH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, rat, pig, rabbit
Immunogen ASAH1 fusion protein Ag28645 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Synonyms ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44-55 kDa
GenBank Accession NumberBC016828
Gene Symbol ASAH1
Gene ID (NCBI) 427
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13510
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

实验方案

Product Specific Protocols
WB protocol for ASAH1 antibody 67092-2-IgDownload protocol
IF protocol for ASAH1 antibody 67092-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols