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验证数据展示

  • WB analysis using 67092-2-Ig

    WB analysis using 67092-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 67092-2-Ig

    IF Staining of MCF-7 using 67092-2-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ASAH1 antibody (67092-2-Ig, Clone: 2B3C7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

ASAH1 Monoclonal antibody

ASAH1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67092-2-Ig

产品说明书

CloneNo. 2B3C7

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig liver tissue, pig brain tissue, rabbit brain tissue, rat brain tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ASAH1 Ready-To-Use IHC Kit. ASAH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67092-2-Ig targets ASAH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat, pig, rabbit
免疫原 ASAH1 fusion protein Ag28645 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 N-acylsphingosine amidohydrolase (acid ceramidase) 1
别名 ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC
计算分子量 44 kDa
观测分子量 44-55 kDa
GenBank蛋白编号BC016828
基因名称 ASAH1
Gene ID (NCBI) 427
RRIDAB_3085035
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13510
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

实验方案

Product Specific Protocols
WB protocol for ASAH1 antibody 67092-2-IgDownload protocol
IF protocol for ASAH1 antibody 67092-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols