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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67092-2-Ig (same clone as 67092-2-PBS)

    WB analysis using 67092-2-Ig (same clone as 67092-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67092-2-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67092-2-Ig (same clone as 67092-2-PBS)

    IF Staining of MCF-7 using 67092-2-Ig (same clone as 67092-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ASAH1 antibody (67092-2-Ig, Clone: 2B3C7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67092-2-PBS in a different storage buffer formulation.

ASAH1 Monoclonal antibody, PBS Only

ASAH1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67092-2-PBS
CloneNo. 2B3C7

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ASAH1 Ready-To-Use IHC Kit. ASAH1 primary antibody included.

产品信息

67092-2-PBS targets ASAH1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat, pig, rabbit
Immunogen ASAH1 fusion protein Ag28645 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Synonyms ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44-55 kDa
GenBank Accession NumberBC016828
Gene Symbol ASAH1
Gene ID (NCBI) 427
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13510
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.