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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ASCT2 Polyclonal antibody

ASCT2 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20350

Print datasheet

Synonyms

SLC1A5, M7VS1, M7V1, Baboon M7 virus receptor, ATBO

验证数据展示

  • IF Staining of mouse small intestine using CL488-20350

    IF Staining of mouse small intestine using CL488-20350

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 ASCT2 antibody (CL488-20350) at dilution of 1:100, Villin antibody (16488-1-AP, Magenta), CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

  • IF Staining of HEK-293 using CL488-20350

    IF Staining of HEK-293 using CL488-20350

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ASCT2 antibody (CL488-20350) at dilution of 1:200.

  • FC experiment of HeLa using CL488-20350

    FC experiment of HeLa using CL488-20350

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ASCT2 (CL488-20350) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse small intestine tissue
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 20350-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20350 targets ASCT2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen ASCT2 fusion protein Ag14182 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 1 (neutral amino acid transporter), member 5
Synonyms SLC1A5, M7VS1, M7V1, Baboon M7 virus receptor, ATBO
Calculated Molecular Weight 541 aa, 57 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC000062
Gene Symbol SLC1A5/ASCT2
Gene ID (NCBI) 6510
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15758
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ASCT2 antibody CL488-20350Download protocol
Standard Protocols
Click here to view our Standard Protocols