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验证数据展示

  • IF Staining of HepG2 using CL594-66884

    IF Staining of HepG2 using CL594-66884

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 ASF1A antibody (CL594-66884, Clone: 2D4A6 ) at dilution of 1:200, CL488-Phalloidin (green).

CoraLite®594-conjugated ASF1A Monoclonal antibody

ASF1A Monoclonal Antibody for IF/ICC
Cat No. CL594-66884

产品说明书

CloneNo. 2D4A6

宿主/亚型

Mouse / IgG3

种属反应性

human, rat

应用

IF/ICC

别名

2D4A6, Anti-silencing function protein 1 homolog A, CCG1 interacting factor A, CCG1-interacting factor A, CGI 98

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66884-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66884 targets ASF1A in IF/ICC applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, rat
免疫原 ASF1A fusion protein Ag1233 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
别名 2D4A6, Anti-silencing function protein 1 homolog A, CCG1 interacting factor A, CCG1-interacting factor A, CGI 98
计算分子量 204 aa, 23 kDa
观测分子量 21-23 kDa
GenBank蛋白编号BC010878
基因名称 ASF1A
Gene ID (NCBI) 25842
RRIDAB_2883631
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y294
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

实验方案

Product Specific Protocols
IF protocol for CL594 ASF1A antibody CL594-66884Download protocol
Standard Protocols
Click here to view our Standard Protocols