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ASF1A/B Polyclonal antibody, PBS Only

ASF1A/B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11011-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse thymus using 11011-1-AP (same clone as 11011-1-PBS)

    WB analysis of mouse thymus using 11011-1-AP (same clone as 11011-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 11011-1-AP (same clone as 11011-1-PBS)

    IHC staining of human lymphoma using 11011-1-AP (same clone as 11011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11011-1-AP (ASF1A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASF1B antibody included
  • IF Staining of HeLa using 11011-1-AP (same clone as 11011-1-PBS)

    IF Staining of HeLa using 11011-1-AP (same clone as 11011-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11011-1-AP (ASF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ASF1B Ready-To-Use IHC Kit. ASF1B primary antibody included.

产品信息

11011-1-PBS targets ASF1A/B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ASF1A/B fusion protein Ag1477 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Synonyms
Calculated Molecular Weight 202 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC010014
Gene Symbol ASF1B
Gene ID (NCBI) 55723
RRIDAB_2059670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVP2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.