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ASH2L Monoclonal antibody, PBS Only

ASH2L Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

rat, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1G1H3

Cat no : 67859-1-PBS

Print datasheet

Synonyms

Bre2, ASH2-like protein, ASH2L2, ASH2L1, ASH2 like protein

验证数据展示

  • WB analysis using 67859-1-Ig (same clone as 67859-1-PBS)

    WB analysis using 67859-1-Ig (same clone as 67859-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

  • WB analysis using 67859-1-Ig (same clone as 67859-1-PBS)

    WB analysis using 67859-1-Ig (same clone as 67859-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67859-1-Ig (same clone as 67859-1-PBS)

    IHC staining of human colon cancer using 67859-1-Ig (same clone as 67859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67859-1-Ig (ASH2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASH2L antibody included
  • FC experiment of HeLa using 67859-1-Ig (same clone as 67859-1-PBS)

    FC experiment of HeLa using 67859-1-Ig (same clone as 67859-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ASH2L (67859-1-Ig, Clone:1G1H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.

产品信息

67859-1-PBS targets ASH2L in Indirect ELISA, IHC, WB applications and shows reactivity with rat, human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity rat, human
Immunogen ASH2L fusion protein Ag3008 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ash2 (absent, small, or homeotic)-like (Drosophila)
Synonyms Bre2, ASH2-like protein, ASH2L2, ASH2L1, ASH2 like protein
Calculated Molecular Weight 628 aa, 69 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC015936
Gene Symbol ASH2L
Gene ID (NCBI) 9070
RRIDAB_2918617
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBL3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C