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ASH2L Monoclonal antibody

ASH2L Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G1H3

Cat No : 67859-1-Ig

Print datasheet

Synonyms

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

验证数据展示

  • WB analysis using 67859-1-Ig

    WB analysis using 67859-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67859-1-Ig

    WB analysis using 67859-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67859-1-Ig

    IHC staining of human colon cancer using 67859-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67859-1-Ig (ASH2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASH2L antibody included
  • FC experiment of HeLa using 67859-1-Ig

    FC experiment of HeLa using 67859-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ASH2L (67859-1-Ig, Clone:1G1H3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67859-1-Ig targets ASH2L in WB, FC, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity Human, rat
Immunogen ASH2L fusion protein Ag3008 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ash2 (absent, small, or homeotic)-like (Drosophila)
Synonyms ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2
Calculated Molecular Weight 628 aa, 69 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC015936
Gene Symbol ASH2L
Gene ID (NCBI) 9070
RRIDAB_2918617
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ASH2L antibody 67859-1-IgDownload protocol
IHC protocol for ASH2L antibody 67859-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols