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验证数据展示

  • FC experiment of HeLa using CL488-67859

    FC experiment of HeLa using CL488-67859

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ASH2L (CL488-67859, Clone:1G1H3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated ASH2L Monoclonal antibody

ASH2L Monoclonal Antibody for FC (Intra)
Cat No. CL488-67859

Print datasheet

CloneNo. 1G1H3

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

FC (Intra)

Synonyms

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67859-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67859 targets ASH2L in FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, rat
Immunogen ASH2L fusion protein Ag3008 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ash2 (absent, small, or homeotic)-like (Drosophila)
Synonyms ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2
Calculated Molecular Weight 628 aa, 69 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC015936
Gene Symbol ASH2L
Gene ID (NCBI) 9070
RRIDAB_2923807
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBL3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ASH2L antibody CL488-67859Download protocol
Standard Protocols
Click here to view our Standard Protocols