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验证数据展示

  • FC experiment of HeLa using CL488-67859

    FC experiment of HeLa using CL488-67859

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ASH2L (CL488-67859, Clone:1G1H3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated ASH2L Monoclonal antibody

ASH2L Monoclonal Antibody for FC (Intra)
Cat No. CL488-67859

产品说明书

CloneNo. 1G1H3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat

应用

FC (Intra)

别名

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67859-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67859 targets ASH2L in FC (Intra) applications and shows reactivity with Human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat
免疫原 ASH2L fusion protein Ag3008 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ash2 (absent, small, or homeotic)-like (Drosophila)
别名 ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2
计算分子量 628 aa, 69 kDa
观测分子量 80 kDa
GenBank蛋白编号BC015936
基因名称 ASH2L
Gene ID (NCBI) 9070
RRIDAB_2923807
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBL3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ASH2L antibody CL488-67859Download protocol
Standard Protocols
Click here to view our Standard Protocols