验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, mouse brain tissue, LNCaP cells, SH-SY5Y cells, rat brain tissue, mouse cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85089-1-RR targets ASIC1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ASIC1 fusion protein Ag25941 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | amiloride-sensitive cation channel 2, neuronal |
| 别名 | ACCN2, ASIC, Amiloride-sensitive cation channel 2, neuronal, Acid-sensing ion channel 1, 242749B11 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | NM_001095 |
| 基因名称 | ASIC1 |
| Gene ID (NCBI) | 41 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P78348 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASIC1(Acid-sensing ion channel 1), also named as ACCN2, BNaC2 or amiloride-sensitive cation channel 2, is a member of the ASICs family. ASICs, an H+-gated subgroup of the epithelial Na+ channel/degenerin (ENaC/DEG) superfamily, are widely expressed throughout the central and peripheral nervous system and triggered by increased extracellular acidification (PMID: 28518134, 27941930). ASIC1 is a key player in acidosis-induced tumor growth and invasiveness. Clinically, alterations of ASIC1 are associated with poor survival in breast cancer (PMID: 26686084).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASIC1 antibody 85089-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |