ASK1 Monoclonal antibody

ASK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67072-1-Ig

产品说明书

CloneNo. 1C3A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat and More (3)

应用

WB, IHC, IF/ICC, ELISA and More (1)

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHSC-T6 cells, HEK-293 cells, Jurkat cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue, mouse heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67072-1-Ig targets ASK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human, Mouse, Rat
文献引用反应性human, mouse, rat
免疫原 ASK1 fusion protein Ag5818 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 5
别名 ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5
计算分子量 155 kDa
观测分子量 110 kDa
GenBank蛋白编号BC054503
基因名称 ASK1
Gene ID (NCBI) 4217
RRIDAB_2882381
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99683
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for ASK1 antibody 67072-1-IgDownload protocol
IHC protocol for ASK1 antibody 67072-1-IgDownload protocol
IF protocol for ASK1 antibody 67072-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer

Authors - Yurou Chen
humanWB

Oncol Rep

ABT‑737 increases cisplatin sensitivity through the ROS‑ASK1‑JNK MAPK signaling axis in human ovarian cancer cisplatin‑resistant A2780/DDP cells

Authors - Xiaoning Li
mouse,ratIF

Cardiovasc Res

Cardiac tumour necrosis factor receptor-associated factor 7 mediates the ubiquitination of apoptosis signal-regulating kinase 1 and aggravates cardiac hypertrophy

Authors - Yan Che
human,ratWB

Aging (Albany NY)

Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF

Authors - Xiao-Lin Jiang
ratWB

Front Endocrinol (Lausanne)

Cangfudaotan decoction inhibits mitochondria-dependent apoptosis of granulosa cells in rats with polycystic ovarian syndrome

Authors - Xiao-Lin Jiang
humanWB

Cells

Orphan Nuclear Receptor Nur77 Mediates the Lethal Endoplasmic Reticulum Stress and Therapeutic Efficacy of Cryptomeridiol in Hepatocellular Carcinoma

Authors - Xudan Li
Loading...